This ONT-specific pipeline enables the detection of native DNA modifications without the need for chemical treatment or bisulfite conversion. It uses aligned ONT sequencing data to compute per-base modification probabilities, providing direct insight into epigenetic marks.

  • Input: Aligned Oxford Nanopore sequencing data (BAM format)

  • Output:

    • calls.tsv containing base-level probabilities for:

      • 5-methylcytosine (5mC)

      • 5-hydroxymethylcytosine (5hmC)

      • 6-methyladenine (6mA)

Ideal for native epigenetic profiling in research and clinical genomics workflows using long-read sequencing.